Desulphation of dextran sulphate during kidney ultrafiltration

Desulphation of dextran sulphate during kidney ultrafiltration.

The renal clearance of [3H]dextran sulphate by the isolated perfused rat kidney was associated with desulphation of the molecule, as demonstrated by ion-exchange and affinity chromatography of material resident in both glomeruli and urine samples. This process also occurred in vivo. The molecular size distribution of glomerular dextran sulphate in the perfused kidney was indistinguishable from ...

Dextran sulphate--a synthetic analogue of heparin.

The structure of the natural anticoagulant, heparin, is gradually being elucidated. As each step forward is made, attempts to synthesize molecules with the biological activity of heparin receive renewed impetus. It is now known (Jorpes, 1939) that heparin is composed of glucuronic acid and glucosamine though the mode of linkage of these two parts is not yet clear. Some of the hydroxyl groups of...

Dextran sulphate induced arthritis in rabbits.

Persistent effects of a short course of intra-articular dextran sulphate, dextrans, or chondroitin sulphate were examined in rabbit knees. Only dextran sulphate produced gross arthritis, associated with high synovial acid phosphatase and beta-glucuronidase activities. Synovial degradative capacity in synovium-cartilage cocultures was increased 2-fold by dextran sulphate and 1.5-fold by chondroi...

Enzymic desulphation of l-serine O[S]-sulphate: the intracellular localization of the enzyme.

Enzymatic desulphation of cerebroside-3-sulphate by chicken brain arylsulphatase A.

IN EARLIER work from this laboratory it was shown that arylsulphatase of chicken brain resembles arylsulphatase A of other animal species in several of its properties but exhibits certain characteristics similar to that of arylsulphatase B (FAROOQUI and BACHHAWAT, 1971). Recently the arylsulphatase A of chicken brain was purified and it was demonstrated that the purified enzyme could desulphate...